dichotomous key microbiology unknown bacteria

DICHOTOMOUS KEY MICROBIOLOGY UNKNOWN BACTERIA PROFESSIONAL

While performing the streak plate method, it was important to spread the bacteria across Prior to streaking, the inoculating loop was sterilized through the Bunsen burnerįlame. Streaks, the third corner with 2-3 streaks, and the fourth with 1-2 streaks across the two nutrientĪgar plates. Streak plate method was performed by inoculating the unknown bacteria number 16 from theīroth tube and at one corner of the plate with about 5-6 streaks, the second corner with 3-4 Nutrient agar plates, an inoculating loop, and a Bunsen burner was needed. To many different tasks in the experiment, it was divided into multiple days. Biochemical testing allows for observing structural differences betweenīacteria that cannot be seen with the naked eye.įirst, broth tube number 16 containing two unknown microorganisms was obtained. For instance, contrasting biochemical tests such asĬatalase testing, mannitol salt agar (MSA), and bile esculin agar may be used to determine an Identification simple (Janda & amp Abbott, 2002).

In a majority of instances,īiochemical test results precisely divided the common species from one another making The key is constructed beginning with whether or not the bacteria is grampositive or gram-negative, then further reduced to whether it is rod-shape or cocci-shaped, andįinally even further reduced to distinctive biochemical tests. Prior to use, the key was created from data in the Center for Disease Control (CDC) Process of identifying an unknown organism. Aĭichotomous key allows for an organized layout of phenotypic characteristics to guide the Keys to keep up with the growing population of new microorganisms (Kellenberger, 2001). There are constantly countless new microorganisms emerging with unknownĬharacteristics which makes it extremely important to use biochemical testing and dichotomous The two, identifying microorganisms can prevent infectious diseases in humans, plants, andĪnimals. Identifying an unknown microorganism can be difficult however, biochemical tests andĭichotomous keys play an important role in helping to alleviate those difficulties. Forecasting, Time Series, and Regression (Richard T.Civilization and its Discontents (Sigmund Freud).

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Dichotomous key microbiology unknown bacteria